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Cellulose Microfibres from Durian Waste using Modified Steam Explosion as Pre-treatment

Authors

  • Akmal Aizuddin Bin Zulkifli Solar Energy Research Institute, Universiti Kebangsaan Malaysia, Malaysia Author
  • Sabariah Kamarudin Solar Energy Research Institute, Universiti Kebangsaan Malaysia, Malaysia Author
  • Norhasnan Sahari Solar Energy Research Institute, Universiti Kebangsaan Malaysia, Malaysia Author
  • Sakhr M. Sultan Solar Energy Research Institute, Universiti Kebangsaan Malaysia, Malaysia Author
  • Norzita Yacob Malaysia Nuclear Agency (Nuclear Malaysia), Malaysia Author
  • Masita Mohammad Solar Energy Research Institute, Universiti Kebangsaan Malaysia, Malaysia Author

DOI:

https://doi.org/10.70917/jcc-2025-005

Keywords:

durian rind, biomass, steam explosion, cellulose microfibres, extraction

Abstract

This study presents a steam explosion (SE) system as an effective pretreatment method for efficiently extracting cellulose microfibres (CMFs) from durian rind, which makes up a significant portion of the fruit (60–75%) and is often discarded as waste. It represents a significant agricultural waste resource of lignocellulosic biomass. SE pretreatment, optimized at 145 °C for 5 minutes on dried durian rind, significantly increased CMF recovery from 34.01% to 63.6%, exceeding typical yields reported in the literature for similar biomass sources. Comprehensive analyses confirmed the quality and structural integrity of the extracted cellulose. X-ray diffraction (XRD) identified the crystalline structure as cellulose type I, while Fourier transform infrared spectroscopy (FTIR) detected characteristic cellulose peaks, affirming its chemical purity. Thermogravimetric analysis (TGA) demonstrated high thermal stability, with decomposition commencing at approximately 340 °C, consistent with pure cellulose. Field emission scanning electron microscopy (FESEM) revealed a smoother surface, refined fibre morphology, and a notable reduction in fibre diameter in SE-treated samples. These findings underscore the effectiveness of the SE system in enhancing CMF yield and purity, offering a sustainable, scalable approach for cellulose extraction from biomass. This work highlights the potential of SE technology as a transformative tool for valorising lignocellulosic waste, supporting its application in biocomposites, biofuels, and nanocellulose-based products. 

References

Abdul Rahman, N.H. et al. (2017) ‘Extraction and Characterization of Cellulose Nanocrystals from Tea Leaf Waste Fibers’, Polymers. Available at: https://doi.org/10.3390/polym9110588.Abelha, P. and Cieplik, M.K. (2021) ‘Evaluation of Steam-Exploded Wood Pellets Storage and Handling Safety in a Coal-Designed Power Plant’, Energy & Fuels, 35(3), pp. 2357–2367. Available at: https://doi.org/10.1021/acs.energyfuels.0c04246.Asada, C. et al. (2015) ‘Conversion of Steam-Exploded Cedar Into Ethanol Using Simultaneous Saccharification, Fermentation and Detoxification Process’, Bioresource Technology, 176, pp. 203–209. Available at: https://doi.org/10.1016/j.biortech.2014.11.039.Auxenfans, T. et al. (2017) ‘Understanding the Structural and Chemical Changes of Plant Biomass Following Steam Explosion Pretreatment’, Biotechnology for Biofuels, 10(1). Available at: https://doi.org/10.1186/s13068-017-0718-z. Birnin-Yauri, A.U. et al. (2016) ‘Influence of kenaf core fiber incorporation on the mechanical performance and dimensional stability of oil palm fiber reinforced poly(lactic acid) hybrid biocomposites’, BioResources, 11(2), pp. 3332–3355. Available at: https://doi.org/10.15376/biores.11.2.3332-3355.Boukir, A., Fellak, S. and Doumenq, P. (2019) ‘Structural Characterization of Argania Spinosa Moroccan Wooden Artifacts During Natural Degradation Progress Using Infrared Spectroscopy (ATR-FTIR) and X-Ray Diffraction (XRD)’, Heliyon, 5(9), p. e02477. Available at: https://doi.org/10.1016/j.heliyon.2019.e02477.Bufalino, L. et al. (2015) ‘How the Chemical Nature of Brazilian Hardwoods Affects Nanofibrillation of Cellulose Fibers and Film Optical Quality’, Cellulose, 22(6), pp. 3657–3672. Available at: https://doi.org/10.1007/s10570-015-0771-3. Chaib, O., Abatzoglou, N. and Achouri, I.E. (2024) ‘Lignocellulosic Biomass Valorisation by Coupling Steam Explosion Treatment and Anaerobic Digestion’, Energies, 17(3), p. 677. Available at: https://doi.org/10.3390/en17030677.Chandra, R.P. et al. (2015) ‘Enhancing Hemicellulose Recovery and the Enzymatic Hydrolysis of Cellulose by Adding Lignosulfonates During the Two-Stage Steam Pretreatment of Poplar’, Acs Sustainable Chemistry & Engineering, 3(5), pp. 986–991. Available at: https://doi.org/10.1021/acssuschemeng.5b00124.Chang, J. et al. (2012) ‘Effect of Steam Explosion Pretreatment and Microbial Fermentation on Degradation of Corn Straw’, Advanced Materials Research, 343–344, pp. 809–814. Available at: https://doi.org/10.4028/www.scientific.net/AMR.343-344.809.Cui, X. et al. (2021) ‘Food Waste Durian Rind-Derived Cellulose Organohydrogels: Toward Anti-Freezing and Antimicrobial Wound Dressing’, Acs Sustainable Chemistry & Engineering, 9(3), pp. 1304–1312. Available at: https://doi.org/10.1021/acssuschemeng.0c07705. Deepa, B. et al. (2015) ‘Utilization of Various Lignocellulosic Biomass for the Production of Nanocellulose: A Comparative Study’, Cellulose, 22(2), pp. 1075–1090. Available at: https://doi.org/10.1007/s10570-015-0554-x. D’Orsi, R. et al. (2023) ‘Isolation of Pure Lignin and Highly Digestible Cellulose From Defatted and Steam-Exploded Cynara Cardunculus’, Acs Sustainable Chemistry & Engineering, 11(5), pp. 1875–1887. Available at: https://doi.org/10.1021/acssuschemeng.2c06356. Dyartanti, E.R. et al. (2019) ‘Pretreatment Ethanol From Cellulosic’, Equilibrium Journal of Chemical Engineering, 3(1), p. 23. Available at: https://doi.org/10.20961/equilibrium.v3i1.43883. Flórez Pardo, L.M., Salcedo Mendoza, J.G. and López Galán, J.E. (2019) ‘Influence Of Pretreatments On Crystallinity And Enzymatic Hydrolysis In Sugar Cane Residues’, Brazilian Journal of Chemical Engineering. scielo.Gamay, R.A.J. et al. (2024) ‘Durian (Durio zibenthinus) waste: a promising resource for food and diverse applications—a comprehensive review’, Food Production, Processing and Nutrition, 6(1), p. 27. Available at: https://doi.org/10.1186/s43014-023-00206-4. Gao, W. et al. (2021) ‘Camellia Oleifera Shell as a Potential Agricultural by-Product for Paper Production’, Bioresources, 16(2), pp. 3734–3745. Available at: https://doi.org/10.15376/biores.16.2.3734-3745.Halysh, V. et al. (2023) ‘Apricot Seed Shells and Walnut Shells as Unconventional Sugars and Lignin Sources’, Molecules, 28(3), p. 1455. Available at: https://doi.org/10.3390/molecules28031455.Han, X. et al. (2007) ‘Effect of Two Methods of Van Soest Fibre Detergent System on Monosaccharide Compositions of Acid Detergent Residues’, Journal of Animal and Feed Sciences, 16(Suppl. 2), pp. 500–505. Available at: https://doi.org/10.22358/jafs/74591/2007.Heikkinen, H. et al. (2014) ‘Impact of Steam Explosion on the Wheat Straw Lignin Structure Studied by Solution-State Nuclear Magnetic Resonance and Density Functional Methods’, Journal of

74 Agricultural and Food Chemistry, 62(43), pp. 10437–10444. Available at: https://doi.org/10.1021/jf504622j. Imlauer-Vedoya, C.M. et al. (2019) ‘Fractionation of Pinus Radiata Wood by Combination of Steam Explosion and Organosolv Delignification’, Maderas Ciencia Y Tecnología, (ahead), p. 0. Available at: https://doi.org/10.4067/s0718-221x2019005000414.Jovičić, N. et al. (2022) ‘Biomass Valorization of Walnut Shell for Liquefaction Efficiency’, Energies, 15(2), p. 495. Available at: https://doi.org/10.3390/en15020495.Kang, Y.K. et al. (2013) ‘SO2‐catalyzed Steam Explosion: The Effects of Different Severity on Digestibility, Accessibility, and Crystallinity of Lignocellulosic Biomass’, Biotechnology Progress, 29(4), pp. 909–916. Available at: https://doi.org/10.1002/btpr.1751.Klunklin, W. et al. (2023) ‘Effect of Bleaching Processes on Physicochemical and Functional Properties of Cellulose and Carboxymethyl Cellulose From Young and Mature Coconut Coir’, Polymers, 15(16), p. 3376. Available at: https://doi.org/10.3390/polym15163376.Kong, F. et al.(2022) ‘Effect of Steam Explosion on Structural Characteristics of Β−Conglycinin and Morphology, Chemical Compositions of Soybean Meal’, Frontiers in Nutrition, 9. Available at: https://doi.org/10.3389/fnut.2022.896664.Kukle, S. et al. (2015) ‘The Effect of Steam Explosion Treatment on Technical Hemp Fibres’, Environment Technology Resources Proceedings of the International Scientific and Practical Conference, 1, p. 230. Available at: https://doi.org/10.17770/etr2011vol1.902. Kumar, P. et al. (2009) ‘Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production’, Industrial & Engineering Chemistry Research, 48(8), pp. 3713–3729. Available at: https://doi.org/10.1021/ie801542g.Lee, H. V, Hamid, S.B.B.O.A.A. and Zain, S.K. (2014) ‘Conversion of Lignocellulosic Biomass to Nanocellulose: Structure and Chemical Process’, The Scientific World Journal, 2014, pp. 1–20. Available at: https://doi.org/10.1155/2014/631013.Liu, Q. et al. (2017) ‘Isolation of High-Purity Cellulose Nanofibers From Wheat Straw Through the Combined Environmentally Friendly Methods of Steam Explosion, Microwave-Assisted Hydrolysis, and Microfluidization’, Acs Sustainable Chemistry & Engineering, 5(7), pp. 6183–6191. Available at: https://doi.org/10.1021/acssuschemeng.7b01108.Liu, Y. et al. (2019) ‘Steam Explosion Treatment of Byproduct Feedstuffs for Potential Use as Ruminant Feed’, Animals, 9(9), p. 688. Available at: https://doi.org/10.3390/ani9090688.Lorenzo-Hernando, A., Martín-Juárez, J. and Bolado-Rodríguez, S. (2018) ‘Study of steam explosion pretreatment and preservation methods of commercial cellulose’, Carbohydrate Polymers, 191, pp. 234–241. Available at: https://doi.org/https://doi.org/10.1016/j.carbpol.2018.03.021.Masrol, S.R. et al. (2017) ‘Effects of Total Chlorine Free (Tcf) Bleaching on the Characteristics of Chemi Mechanical (Cmp) Pulp and Paper From Malaysian Durian (Durio Zibethinus Murr.) Rind’, Jurnal Teknologi, 79(4). Available at: https://doi.org/10.11113/jt.v79.9803.Mohamood, N.F.A.-Z.T., Halim, A.H.A. and Zainuddin, N. (2021) ‘Carboxymethyl Cellulose Hydrogel From Biomass Waste of Oil Palm Empty Fruit Bunch Using Calcium Chloride as Crosslinking Agent’, Polymers, 13(23), p. 4056. Available at: https://doi.org/10.3390/polym13234056.Mulat, D. et al. (2018) ‘Enhancing Methane Production From Lignocellulosic Biomass by Combined Steam-Explosion Pretreatment and Bioaugmentation With Cellulolytic Bacterium Caldicellulosiruptor Bescii’, Biotechnology for Biofuels, 11(1). Available at: https://doi.org/10.1186/s13068-018-1025-z. Nader, S. et al. (2022) ‘A Low-Cost Environmentally Friendly Approach to Isolate Lignin Containing Micro and Nanofibrillated Cellulose From Eucalyptus Globulus Bark by Steam Explosion’, Cellulose, 29(10), pp. 5593–5607. Available at: https://doi.org/10.1007/s10570-022-04632-4. Narkesabad, Z.S., Rafiee, R. and Jalilnejad, E. (2023) ‘Experimental Study on Evaluation and Optimization of Heavy Metals Adsorption on a Novel Amidoximated Silane Functionalized Luffa Cylindrica’, Scientific Reports, 13(1). Available at: https://doi.org/10.1038/s41598-023-30634-8. Ngabura, M. et al. (2018) ‘Utilization of renewable durian peels for biosorption of zinc from wastewater’, Journal of Environmental Chemical Engineering, 6(2), pp. 2528–2539. Available at: https://doi.org/10.1016/j.jece.2018.03.052.Obame, S.N. et al.(2019) ‘Homolytic and Heterolytic Cleavage of Β-Ether Linkages in Hardwood Lignin by Steam Explosion’, Journal of Agricultural and Food Chemistry, 67(21), pp. 5989–5996. Available at: https://doi.org/10.1021/acs.jafc.9b01744.Obeng, A.K., Premjet, D. and Premjet, S. (2021) ‘Improved Glucose Recovery From Durian Peel by Alkaline-Catalyzed Steam Pretreatment’, Peerj, 9, p. e12026. Available at: https://doi.org/10.7717/peerj.12026.

75 Olsson, J. et al. (2019) ‘Sequential Fractionation of the Lignocellulosic Components in Hardwood Based on Steam Explosion and Hydrotropic Extraction’, Biotechnology for Biofuels, 12(1). Available at: https://doi.org/10.1186/s13068-018-1346-y. Ouyang, X., Chen, L., Zhang, S., Yuan, Q., Wang, W, et al. (2018) ‘Effect of Simultaneous Steam Explosion and Alkaline Depolymerization on Corncob Lignin and Cellulose Structure’, Chemical and Biochemical Engineering Quarterly, 32(2), pp. 177–189. Available at: https://doi.org/10.15255/cabeq.2017.1251.Payus, C.M. et al. (2021) ‘Durian husk wastes as low-cost adsorbent for physical pollutants removal: groundwater supply’, Materials Today: Proceedings, 42, pp. 80–87. Available at: https://doi.org/10.1016/j.matpr.2020.10.006.Pérez-Aguilar, H., Ruzafa-Silvestre, C. and Arán-Aı́s, F. (2022) ‘Effect of Processing Time of Steam-Explosion for the Extraction of Cellulose Fibers From Phoenix Canariensis Palm Leaves as Potential Renewable Feedstock for Materials’, Polymers, 14(23), p. 5206. Available at: https://doi.org/10.3390/polym14235206.Pielhop, T. et al. (2016) ‘Steam Explosion Pretreatment of Softwood: The Effect of the Explosive Decompression on Enzymatic Digestibility’, Biotechnology for Biofuels, 9(1). Available at: https://doi.org/10.1186/s13068-016-0567-1. Qi, B., Liang, X. and Luo, J. (2021) ‘Comparison of Corn Stover Pretreatments With Lewis Acid Catalyzed Choline Chloride, Glycerol and Choline Chloride-Glycerol Deep Eutectic Solvent’, Polymers, 13(7), p. 1170. Available at: https://doi.org/10.3390/polym13071170.Sain, M. and Panthapulakkal, S. (2006) ‘Bioprocess preparation of wheat straw fibers and their characterization’, Industrial Crops and Products, 23(1), pp. 1–8. Available at: https://doi.org/10.1016/j.indcrop.2005.01.006.Sainorudin, M.H. et al. (2021) ‘Investigation of the Structural, Thermal and Morphological Properties of Nanocellulose Synthesised from Pineapple Leaves and Sugarcane Bagasse’, Current Nanoscience, 18(1), pp. 68–77. Available at: https://doi.org/10.2174/1573413717666210216115609.Sebestyén, Z. et al. (2013) ‘Thermal Behavior of Native, Washed and Steam Exploded Lignocellulosic Biomass Samples’, Journal of Analytical and Applied Pyrolysis, 101, pp. 61–71. Available at: https://doi.org/10.1016/j.jaap.2013.02.011.Segal, L. et al. (1959) ‘An Empirical Method for Estimating the Degree of Crystallinity of Native Cellulose Using the X-ray Diffractometer’, Textile Research Journal, 29(10), pp. 786–794. Available at: https://doi.org/10.1177/004051755902901003.Seidel, C.-M. et al. (2019) ‘Two-Stage Steam Explosion Pretreatment of Softwood With 2-Naphthol as Carbocation Scavenger’, Biotechnology for Biofuels, 12(1). Available at: https://doi.org/10.1186/s13068-019-1373-3. Serrano-Martínez, V.M. et al. (2024) ‘Steam Explosion-Based Method for the Extraction of Cellulose and Lignin From Rice Straw Waste’, Applied Sciences, 14(5), p. 2059. Available at: https://doi.org/10.3390/app14052059.Setyaningrum, H. et al. (2021) ‘Durian Rind Micro Composter Model: A Case of Kampung Durian, Ngrogung, Ponorogo, Indonesia’, E3s Web of Conferences, 226, p. 21. Available at: https://doi.org/10.1051/e3sconf/202122600021.Sharma, N. et al. (2023) ‘Rice Straw-Derived Cellulose: A Comparative Study of Various Pre-Treatment Technologies and Its Conversion to Nanofibres’, Scientific Reports, 13(1). Available at: https://doi.org/10.1038/s41598-023-43535-7. Silveira, M.H.L. et al. (2015) ‘Current Pretreatment Technologies for the Development of Cellulosic Ethanol and Biorefineries’, Chemsuschem, 8(20), pp. 3366–3390. Available at: https://doi.org/10.1002/cssc.201500282.Steinbach, D. et al. (2020) ‘Is Steam Explosion a Promising Pretreatment for Acid Hydrolysis of Lignocellulosic Biomass?’, Processes, 8(12), p. 1626. Available at: https://doi.org/10.3390/pr8121626.Szadkowski, J. et al. (2023) ‘Effect of Steam Explosion Pretreatment of Fast-Growing Poplar (Populus Deltoides X Maximowiczii) Wood on Selected Properties of Structural Substances’. Available at: https://doi.org/10.21203/rs.3.rs-3053466/v1.Then, Y.Y. et al. (2014) ‘Surface modifications of oil palm mesocarp fiber by superheated steam, alkali, and superheated steam-alkali for biocomposite applications’, BioResources, 9(4), pp. 7467–7483. Available at: https://doi.org/10.15376/biores.9.4.7467-7483.Thorogood, C.J. et al. (2022) ‘The king of fruits’, PLANTS, PEOPLE, PLANET, 4(6), pp. 538–547. Available at: https://doi.org/10.1002/ppp3.10288.

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2025-05-30 — Updated on 2026-10-09

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Cellulose Microfibres from Durian Waste using Modified Steam Explosion as Pre-treatment. (2026). Journal of Climate Change, 11(1), 18. https://doi.org/10.70917/jcc-2025-005 (Original work published 2025)